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產(chǎn)地 | 國(guó)產(chǎn)、進(jìn)口 |
品牌 | 上海滬崢 |
保存條件 | -20 °C |
貨號(hào) | HZK-100288 |
應(yīng)用范圍 | WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 |
抗體名 | 鏈霉素圄體 |
克隆性 | 是 |
靶點(diǎn) | 電詢 |
適應(yīng)物種 | 人、変鼠、小鼠、兔、雞、豬等 |
形態(tài) | 液體 |
宿主 | 兔、小鼠、羊 |
亞型 | IgG |
標(biāo)識(shí)物 | 詳見(jiàn)說(shuō)明書(shū) |
濃度 | 99% |
免疫原 | 詳見(jiàn)說(shuō)明 |
鏈霉素抗體廠家直銷,上海滬崢質(zhì)量穩(wěn)定、價(jià)格可靠,全國(guó)免費(fèi)運(yùn)輸。
上海滬崢專業(yè)供應(yīng)各類一抗、二抗、標(biāo)記二抗、抗原等多種抗體產(chǎn)品,主要用于石蠟切片免疫組化,冰凍切片免疫組化,Elisa,WB,免疫熒光等科研實(shí)驗(yàn)領(lǐng)域,抗體的相關(guān)標(biāo)記:HRP,Biotin,Gold,RBITC,AP,FITC,Cy3,Cy5,Cy5.5,Cy7,PE,PE-Cy3,PE-Cy5,PE-Cy5.5,PE-Cy7,APC,Alexa Fluor 350,Alexa Fluor 488,Alexa Fluor 555,Alexa Fluor 647等,
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鏈霉素抗體產(chǎn)品信息:
中文名稱 鏈霉素抗體
產(chǎn)品類型藥物與化合物抗體
研究領(lǐng)域免疫學(xué) 藥物及化合物
抗體來(lái)源Rabbit
克隆類型Polyclonal
交叉反應(yīng) streptomycin
產(chǎn)品應(yīng)用ELISA=1:500-1000 (石蠟切片需做抗原修復(fù))
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量1.45738kDa
性 狀Lyophilized or Liquid
濃 度1mg/1ml
免 疫 原KLH conjugated streptomycin
亞 型IgG
純化方法affinity purified by Protein A
儲(chǔ) 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
產(chǎn)品介紹background:
Streptomycin is an antibiotic produced by soil bacteria of the genus Streptomyces and is active against both gram positive and gram negative bacteria, including species resistant to other antibiotics, eg some streptococci, penicillin resistant staphylococci, and bacteria of the genera Proteus and Pseudomonas. Originally isolated by Selman A. Waksman and Albert Schatz in 1947, streptomycin is effective against tubercle bacilli and is a mainstay of tuberculosis therapy. Because streptomycin resistant tubercle bacilli emerge during treatment, the antibiotic is usually used in combination with one or more of the drugs isoniazid, ethambutol, and aminosalicylic acid. Streptomycin acts by inhibiting protein synthesis and damaging cell membranes in susceptible microorganisms. Possible side effects include injury to the kidneys and nerve damage that can result in dizziness and deafness.
鏈霉素抗體注意事項(xiàng):
1.抗體保存原則:避免反復(fù)凍融
2.短期保存的抗體:如抗體幾周內(nèi)就會(huì)使用,收到后推薦在2-8℃保存,2-8℃短暫保存數(shù)周對(duì)抗體活性沒(méi)有影響。
3.長(zhǎng)期保存的抗體:如抗體需超過(guò)1個(gè)月后才會(huì)使用,請(qǐng)酌情分裝后于-20℃保存。
4.建議:將抗體保存在冰箱里層,避免溫度變化造成反復(fù)凍融。
*避免將抗體保存在自動(dòng)除霜冰箱中。
反復(fù)凍融過(guò)程中,冰晶會(huì)對(duì)抗體的空間結(jié)構(gòu)造成破壞,導(dǎo)致蛋白變性形成多聚體,從而降低抗體的結(jié)合能力,加快抗體降解速度。
鏈霉素抗體反應(yīng)特點(diǎn)
1.高度特異性,特異性是抗原抗體反應(yīng)的最主要特征,特定抗原決定簇只與特定抗體結(jié)合;
2.可逆性:抗原與抗體結(jié)合形成抗原抗體復(fù)合物的過(guò)程是一種動(dòng)態(tài)平衡, 其反應(yīng)式為:Ag+AbAg·Ab;
3.適當(dāng)濃度和比例;
4.敏感性:在測(cè)定血清中某一物質(zhì)的含量時(shí),化學(xué)比色法的敏感度為mg/ml水平,酶反應(yīng)測(cè)定法
5.酸堿度:合適的pH為6-8。
6.溫度:37℃通常是抗原體反應(yīng)最適溫度。
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